DEFINITION
1.BIOCHEMICAL OXYGEN DEMAND (BOD):
the amount of oxygen that would be consumed if all the organic matter in 1 liter of water was oxidize by the bacteria.
2. BIOCONTROL
the use of biological methods for controlling plant disease and pets.
3. BIOFERTILISERS
the organisms the enrich the nutrient quality of the soil.
4. BIOGAS
The mixture of gasses (mainly CH4,CO2) produced by the microbial activity and which can be used as fuel.
5. BT COTTON
A variety of cotton which is incorporated with BT gene and it is resistant for insects pests.
6. CLOT BUSTER
The microbial product for removing clots from the blood vessels of the patients
who have undergone myocardial infraction leading to heart attack.
7. FERMENTATION
The process of anaerobic respiration in which the complex molecules incompletely brakes into simple ones
by the microbial action.
8. MYCRORRHIZA
A symbiotic relation between fungal hyphal and roots of the tree (Higher plant)
9. PEST
Organism that destroys crop or its products is known as pest.
10. SEWAGE
The waste- water containing large amount of organic matter and microbes.
Microbes are found everywhere like and its general characteristics are;
a. Thermal vents of geyser (Temp. above 1000c)
b. Deep in soil.
c. Under snow.
d. Diverse and found in Protozoa, Bacteria, Fungi, Virus, Viroids, Prions (Proteinaceous infectious agents)
e. Useful in Antibiotics.
f. Harmful: Cause different diseases.
In Household Products
1.Everyday : Lactobacillus (LAB) Lactic acid Bacteria – form curd from milk.
2.Increase Vitamin B12
3.Check disease causing microbes in our stomach.
4.Fermentation of dough for dosa, idli (CO2 produced)
5.Making bread –Baker‘s yeast.Saccharomyces cerevisiae.
6.Cheese making (eg.Swiss cheesse by Propionibacterium sharmanii, Roquefort cheese by fungi.)
In Industrial Products
Beverages and antibiotics.
Fermentors :Large vessels for growing microbes.
Fermented Beverages
Beverages like wine, bear, whisky, Brandy, Rum (Saccharomyces cerevisiae)
Malted cereals and fruit juices used to produce ethanol, wine and beer produced without distillation.
Whisky, brandy, rum are produced after distillation.
Antibiotics
Penicillin produced by Alexander Fleming from Penicillium notatum while working with Staphylococci Earnest Chain and Howard Plorey
awarded Nobel Prize in 1945 for establishing Penicillin as an effective antibiotic.
Uses:
Treat diseases like plague, whooping cough, diphtheria, leprosy.
Chemicals:
Enymes and other Bioactivities Molecules.
Uses:
a.Aspergillus niger for production of Citric Acid.
b.Aspergillus niger for production of Citric Acid.
c.Acetobacter aceti for production of Acetic Acid.
d.Clostridium butylicum for production of Butyric Acid.
e.Lactobacillus for production of Lactic acid.
f. Lipases used in detergents to remove oil strains from Laundry.
g.Pectinases and Proteases to clarify bottled jucies.
h.Streptokinase (from Streptococcus) as clot buster in patients with myocardial infraction (heartattack).
i.Cyclosporin A:
An immuno-suppresant used in organ transplant patients (produced by Trichoderma polysporum)
Statins produced by yeast Monascus purpureus used as blood, cholesterol lowering agent.
Microbes in sewage Treatment
Why treatment?
The answer is:
a. Major component of waste water, human excreta.
b. Waste water sewage.
c. Cannot be disposed directly into rivers and streams.
Where & how ?
Before disposal sewage treated in sewage treatment plants (STPs)
Treatment done in two stages.
Primary :
Physical removal of particles large and small by filtration and sedimentation.
Solids – primary sludge.
Supernatant – effluent.
Secondary :
Primary effluent taken to large aeration tanks.
Agitated mechanically and air pumped into it.
Aerobic microbes form masses with fungal filaments flocs.
Microbes consume organic matter in effluent for growth.
BOD ( Biological oxygen demand) reduced.
Passed into settling tank.
Bacterial flocs sedimented (activated sludge)
Small part of activated sludge used as inoculums in aeration tank.
Major part pumped into large anaerobic sludge digesters.
Anaerobic bacteria digest bacteria and fungi.
Bacteria produce gases such as methane, hydrogen sulphide and CO2 – Biogas.
Secondary effluent released into rivers and streams.
No man made technology available till date.
Untreated sewage if released into rivers causes pollution.
Biogas plant
Concrete tank 10- 15 meters deep, slurry or dung fed.
Floating cover placed above rises as biogas content rises.
Connecting pipe for supply of biogas.
Used for cooking and lighting.
Microbes as Biocontrol Agents
Natural predation better method.
No of pests kept in check, not totally eradicated.
Food chains not disturbed
Eg. Ladybird and Dragon flies useful to get rid of aphids and mosquitoes.
Bacillus thuringiensis (Bt) used to control butterfly caterpillar.
Mode of spores operation.
Available is sachets, mixed with water and sprayed on plants.
Eaten by insect larva.
Toxin released in gut kills larvae.
Now Bt toxin genes introduced into plants resistant to insect pests.
e.g. Bt cotton.
Tungus trichoderma now being developed.
Nucleo polyhedrovirus good for narrow spectrum insecticide applications.
No negative impacts on plants, mammals, birds, fish or target insects.
Microbes as Biofertilizers
Chemical fertilizers major pollutant.
Switch to organic farming and use of biofertilizers need of the time.
Main sources of biofertilizers. Bacteria, Fungi & Cyanobacteria.
Eg Rhizobium present in roots of leguminious plants fix atmospheric nitrogen into usable organic form.
Azospirillium and Azotobacter free living bacteria – fix atmospheric Nitrogen.
Symbiotic Associations:
For example, Genus Glomus sp. form mycorrhiza.
Fungal symbiont absorbs phosphorus from soil and passes it to plant.
Plants show:
resistance to root – borne pathogens.
Tolerance to salinity and drought.
Increase in growth and development.
Cynobacteria:
Autotrophic – fix atmospheric nitrogen
Imp.biofertilizer. e.g. Anabaena, Nostoc, Oscillatoria.
Blue green algae – increase fertility by adding organic matter.
No. of biofertilizers are commercially available.
Microbes For production of biodegradable plastics
Biodegradable plastic, e.g. polyhydroxybutyrate (PHB) is being produced commercially by
fermentation with the bacterium Alcaligenes eutrophus.
Production of PHB may be easily achieved in tree plants like populous, where PHB can be extracted from leaves.
Other main drawback of bacterial PHB is its high production cost,
making it substantially very expensive than synthetic plastics.
Microbes As edible vaccines
The genes encoding the antigenic proteins of virus and bacteria can be isolated from the pathogens and expressed in plants.
Such transgenic plants or their tissues producing antigens can be eaten for vaccination/immunization (edible vaccines).
The expression of such antigenic proteins in crops like banana and tomato are useful for immunization of humans since banana and
tomato fruits can be eaten raw. Example: cholera and hepatitis B vaccine.
Procedure of sewage treatment in STP
a) Primary treatment(physical )
b) Secondary treatment(biological)
Effluent loaded in large aeration tank, Agitation & rapid growth of aerobic microbes (flocs) ,Consumes organic matter ,reduces BOD,
Effluent passed to settling tank, Flocs sediments form activated
sludge(A.S.) Poured into sludge digester(small amount of A.S. used as inoculum) Filtration & sedimentation.
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